Alkaline phosphatase (ALP) Alkalinephosphatase, referred to as ALP or ALKP) (EC3.1.3.1) is a class of water chemicalhydrolase, which can remove phosphate groups on nucleotides, proteins, alkaloids and other molecules and perform dephosphorylation. It is most effective in alkaline environment, so it is named Alkalinephosphatase.Alkaline phosphatase (ALP) is an exoenzyme of osteoblasts, and its expression activity is an obvious feature of osteoblast differentiation. The main physiological function of alkaline phosphatase in the body is that Chemicalbook hydrolyzes phosphate esters in the process of osteogenesis to provide necessary phosphate for the deposition of hydroxyapatite. At the same time, it hydrolyzes pyrophosphate to relieve its inhibitory effect on bone salt formation and is conducive to osteogenesis.Alkaline phosphatase is an enzyme that can dephosphorylate the corresponding substrate, that is, the phosphate group on the substrate molecule is removed by hydrolysis of phosphate monoester, and the phosphate ion and free hydroxyl group are generated. Such substrates include nucleic acids, proteins, alkaloids, etc. The process of removing the phosphate group is called dephosphorylation or dephosphorylation. Alkaline phosphatases are the Chemicalbook of phosphatases. Phosphatases act in contrast to kinases, which are phosphorylase enzymes that use energy molecules, such as ATP, to add phosphate groups to their corresponding substrate molecules. Alkaline phosphatase has the maximum activity in alkaline environment, and its optimal pH is 8.0 for bacterial ALP and 8.5 for bovine ALP.
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