HEPES Chemical Properties |
Melting point | 234-238 °C |
density | 1.07 g/mL at 20 °C |
refractive index | n 20/D 1.339 |
storage temp. | 2-8°C |
solubility | H 2 O: 1 M at 20 °C, clear, colorless |
form | powder |
color | White |
PH | 5.0-6.5 (1Maqueous solution) |
PH Range | 7.0 - 7.6 |
pka | 7.5(at 25ºC) |
Water Solubility | Soluble |
λmax |
λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04 |
Merck | 14,4654 |
BRN | 883043 |
Stability: | Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture. |
InChIKey | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
CAS DataBase Reference | 7365-45-9(CAS DataBase Reference) |
EPA Substance Registry System | 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)- (7365-45-9) |
Safety Information |
Hazard Codes | Xi |
Risk Statements | 36/37/38 |
Safety Statements | 24/25-22-36-26 |
WGK Germany | 1 |
RTECS | TL6809000 |
TSCA | Yes |
HS Code | 29335995 |
MSDS Information |
HEPES Usage And Synthesis |
Chemical Properties | White crystalline powder |
Uses | Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability. |
Uses | Biological buffer. |
Definition | ChEBI: A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2. |
Biological Activity | Multi purpose buffer used in biological research. |
Purification Methods | Crystallise the acid from hot EtOH and water. It is a useful buffer. [Beilstein 23 V 376.] |